rabbit anti cd107a lamp1  (Proteintech)

Journal: Autophagy

Article Title: Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregates

doi: 10.1080/15548627.2024.2394306

Figure Lengend Snippet: Phosphorylation of TAX1BP1 is required for localization to lysosomes. (A) Immunofluorescence assays of TAX1BP1 KO HeLa cells transfected with Flag-TAX1BP1 WT, 10A or 3SD (S254D, S593D and S666D) and 24 h later transfected with 2.5 µg/ml poly(I:C) for 6 h in the presence of 20 µm leupeptin. Scale bar: 10 µm. (B) Pearson’s correlation coefficient was calculated to measure colocalization between TAX1BP1 and LAMP1 in 8-12 cells randomly selected from each sample. Unpaired Student’s t -test, *** p < 0.001, n.s. = not significant.

Article Snippet: Antibodies used in immunofluorescence experiments were anti-TAX1BP1 (Cell Signaling Technology, 5105S), anti-SQSTM1/p62 (MBL Life Science, PM045), anti-ubiquitin (Cayman, 14220), anti-LAMP1 (Sino Biological, 11215-R107), Alexa Fluor 488 goat anti-mouse IgG (ThermoFisher Scientific, A11029) and Alexa Fluor 594 goat anti-rabbit IgG (ThermoFisher Scientific, A11037).

Techniques: Immunofluorescence, Transfection

Journal: Pharmaceuticals

Article Title: LAMP1 as a Target for PET Imaging in Adenocarcinoma Xenograft Models

doi: 10.3390/ph18081122

Figure Lengend Snippet: Integrated analysis of single-cell RNA sequencing and spatial transcriptomics reveals elevated LAMP1 expression in MDSCs and CAFs with enrichment in tumor regions compared to adjacent normal tissue. ( A ) K-means clustering analysis of single-cell RNA sequencing data highlights distinct cellular populations within the tumor microenvironment. Normalized log2 expression levels of CD68 ( B ), LAMP1 ( C ), CD74 ( D ), and FXYD3 ( E ) across all identified cell populations. ( F ) Quantitative analysis shows high expression of LAMP1 in cancer cells as well as macrophages and CAFs, which are prominent components of the tumor microenvironment. ( G ) Subtype clustering of macrophages identifies functional subsets, allowing for finer delineation of LAMP1 expression within specific macrophage populations. ( H ) Normalized log2 expression of LAMP1 within macrophage subtypes highlights differential expression patterns. ( I ) LAMP1 expression was significantly elevated in the MDSC subtype of macrophages. ( J ) Combined analysis of tumor marker expression within the spatial transcriptomics dataset reveals high expression of FXYD3, human epidermal growth factor receptor 2 (HER2), estrogen receptor (ER), MKI67, topoisomerase II alpha (TOP2A), and epithelial cell adhesion molecule (EPCAM). ( K ) Annotation of cancer cell regions in breast cancer tissue highlights tumor-specific regions. Spatial expression maps show CD74 ( L ), CD68 ( M ), and LAMP1 ( N ) in breast cancer tissue, with notable localization in cancer cells and tumor-associated immune cells. ( O ) Spatial co-expression analysis of CD74, CD68, and LAMP1 with a magnified view and ( P ) comparative analysis of LAMP1 expression between cancerous regions and adjacent normal tissue confirms a significant elevation of LAMP1 in tumor regions. ***: p < 0.001.

Article Snippet: Rabbit anti-human LAMP1 mAb (Novus Biological, Centennial, CO, USA, Cat# NBP2-89844, RRID# AB_3442448) was conjugated to p-SCN-Bn-deferoxamine (DFO) chelator (Macrocyclics, Plano, TX, USA, CAT# B-705) before radiolabeling with 89 Zr according to the standard methods [ ].

Techniques: RNA Sequencing, Expressing, Functional Assay, Quantitative Proteomics, Marker

Journal: Pharmaceuticals

Article Title: LAMP1 as a Target for PET Imaging in Adenocarcinoma Xenograft Models

doi: 10.3390/ph18081122

Figure Lengend Snippet: Assessment of LAMP1 expression in human pan-cancer samples and tumor-bearing murine model. ( A ) Representative immunofluorescence staining of LAMP1 (red channel) in major carcinomas and their respective normal tissue. Nuclei are stained with DAPI (blue channel). ( B ) The overall LAMP1 fluorescent signal intensity in tumor cores (red) versus normal cores (green). ( C ) Organ-based comparison of fluorescent signal intensity demonstrated significantly higher LAMP1 fluorescence in prostate, pancreas, colon, breast, and uterine endometrium carcinomas (red) compared to normal tissue (green). *, p < 0.05; **, p < 0.001; ****, p < 0.0001.

Article Snippet: Rabbit anti-human LAMP1 mAb (Novus Biological, Centennial, CO, USA, Cat# NBP2-89844, RRID# AB_3442448) was conjugated to p-SCN-Bn-deferoxamine (DFO) chelator (Macrocyclics, Plano, TX, USA, CAT# B-705) before radiolabeling with 89 Zr according to the standard methods [ ].

Techniques: Expressing, Immunofluorescence, Staining, Comparison, Fluorescence

Journal: Pharmaceuticals

Article Title: LAMP1 as a Target for PET Imaging in Adenocarcinoma Xenograft Models

doi: 10.3390/ph18081122

Figure Lengend Snippet: LAMP1 protein expression in tumor-bearing murine model. ( A ) LAMP1 positive cell population across major normal organs and tumor (cell surface and total) in tumor-bearing mouse model. ( B ) LAMP1 positive cell population proportion across CD45- and CD45+ cells in tumor tissue. ( C ) Total cell population of CD45 + LAMP1+ cell in major normal organs.

Article Snippet: Rabbit anti-human LAMP1 mAb (Novus Biological, Centennial, CO, USA, Cat# NBP2-89844, RRID# AB_3442448) was conjugated to p-SCN-Bn-deferoxamine (DFO) chelator (Macrocyclics, Plano, TX, USA, CAT# B-705) before radiolabeling with 89 Zr according to the standard methods [ ].

Techniques: Expressing

Journal: Pharmaceuticals

Article Title: LAMP1 as a Target for PET Imaging in Adenocarcinoma Xenograft Models

doi: 10.3390/ph18081122

Figure Lengend Snippet: LAMP1 PET/CT imaging detects tumors in a tumor-bearing murine model at different time points. ( A ) Representative 89 Zr-DFO-LAMP1 and 89 Zr-DFO-IgG PET/CT images of MDA-MB-231 and Caco2 on day 1, day 3, and day 7. Red arrows point at the tumor. ( B ) Tumor SUV max uptake of LAMP1 over the course of the experiment in MDA-MB-231 (white) and Caco2 (gray) reflected a sustained increase in tumor tracer localization. ( C ) Tumor SUV max of 89 Zr-DFO-LAMP-1 (red) in comparison to blood pool (white) in MDA-MB-231 (uptake in the heart region was used as a reference for the blood pool). ( D ) Tumor SUV max of 89 Zr-DFO-LAMP1 (red) in comparison to blood pool (white) in Caco-2 (uptake in the heart region was used as a reference for the blood pool). ( E ) Comparing the uptake of 89 Zr-DFO-IgG (white) and 89 Zr-DFO-LAMP1 (gray) in two MDA-MB-231 and Caco-2 during different time points in both models. ****, p < 0.0001.

Article Snippet: Rabbit anti-human LAMP1 mAb (Novus Biological, Centennial, CO, USA, Cat# NBP2-89844, RRID# AB_3442448) was conjugated to p-SCN-Bn-deferoxamine (DFO) chelator (Macrocyclics, Plano, TX, USA, CAT# B-705) before radiolabeling with 89 Zr according to the standard methods [ ].

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Comparison